vps34 rabbit polyclonal antibody  (Proteintech)

Journal: Brain Pathology

Article Title: AMBRA1, a novel α‐synuclein‐binding protein, is implicated in the pathogenesis of multiple system atrophy

doi: 10.1111/bpa.12461

Figure Lengend Snippet: Western blot analysis of total brain lysates from MSA patients and normal controls. Raw data for the levels of ULK1, ULK2, AMBRA1, TRAF6, beclin1, VPS34, p62, LC3 and actin in the cerebellar white matter of patients with MSA (n = 5) and normal controls (n = 5) (A). In patient with MSA, there were significantly increased expression levels of ULK1 (B), ULK2 (C) and AMBRA1 (D). On the other hand, TFAF6 was significantly decreased in patients with MSA (E). No significant difference in the levels of beclin1 (F), VPS34 (G), p62 (H) and LC3‐II relative to LC3‐I (I) between MSA patients and normal controls. *P < 0.05; **P < 0.01.

Article Snippet: Antibodies Rabbit polyclonal antibodies against ULK1 (Thermo Fisher Scientific, Waltham, MA), ULK2 (Thermo Fisher Scientific), VPS34 (Thermo Fisher Scientific), beclin1 (Novus Biologicals, Littleton, CO), AMBRA1 (ProSci‐Incorporated, Poway, CA), AMBRA1 (Cell Signaling Technology, Danvers, MA), AMBRA1 (Proteintech Inc., Chicago, IL), elongin B (Abcam, Cambridge, MA), p62 (MBL, Nagoya, Japan), microtubule‐associated protein light chain 3 (LC3) (Sigma, St. Louis, MO), TNF receptor associated factor 6 (TRAF6) (Sigma) and β‐actin (Sigma), goat polyclonal antibody against Bip (Santa Cruz, Dallas, TX), rabbit monoclonal antibody against phosphorylated α‐syn (p‐α‐syn) (Abcam) and Myc (Cell Signaling Technology), and mouse monoclonal antibodies against α‐syn (Abcam, 4D6; Abcam, syn211), p‐α‐syn (Wako, Osaka, Japan; #64), dynein intermediate chain (Abcam), Flag (Sigma), RGHHHHHH His (RH) (QIAGEN, Hilden, Germany), Myc (Cell Signaling Technology) and HaloTag (Promega, Madison, WI) were utilized as primary antibodies.

Techniques: Western Blot, Expressing